- 1.1.1 The objectives and development of cell biology
- 1.2.1 Microscope and Cell theory
- 1.3.1 Major events in cell biology
- 2.1.1 cell culture
- 2.2.1 Monoclonal antibodies
- 2.3.1 Fluorescence Microscopy
- 2.4.1 Flow cytometry
- 2.5.1 Spinning Disk Confocal Microscopy
- 2.6.1 FRAP and FRET
- 2.7.1 RNA interference
- 3.1.1 The structrures of the plasmic membrane
- 3.2.1 The lipid bilayer Composition and structural organization
- 3.3.1 Forms of lipid molecules exist in water
- 3.4.1 Lipids Movement in biomembrane
- 3.5.1 Membrane Proteins
- 3.6.1 Synthesis of Phospholipids and Intracellular Movement
- 5.1.1 Overview of transmembrane transport
- 5.2.1 Facilitated Transport of Glucose and Water
- 5.3.1 ATP –powered pumps
- 5.4.1 Na+k+ pump
- 5.5.1 Ca2+ pump
- 5.6.1 K+ channel and the Resting membrane potencial
- 5.7.1 Cotransport by Symporters and Antiporters
- 5.8.1 Transcellular transport
- 6.1.1 Overview of aerobic oxidation
- 6.2.1 Structure of Mitochondria
- 6.3.1 Mitochondrial Oxidation of Fatty Acids Generates ATP
- 6.4.1 The Electron Carriers and Electron Transport Chain
- 6.5.1 The Electron Transport and Proton Motive Force
- 6.6.1 Chemiosmotic theory and Endosymbiont hypothesis
- 6.7.1 Harnessing the Proton-Motive Force to synthesize ATP
- 6.8.1 Electron Transport and Reactive Oxygen Species (ROS)
- 7.1.1 Targeting Proteins to and Across the ER Membrane
- 7.2.1 Insertion of single-pass Proteins into the ER
- 7.3.1 Insertion of Multipass Proteins into the ER
- 7.4.1 Protein Glycosylation and Folding in the ER
- 7.5.1 Formation of disulfide bonds in the ER
- 7.6.1 Protein Quality Control in the ER
- 7.7.1 Targeting of Proteins to Mitochondria
- 7.8.1 Targeting of Peroxisomal Proteins
- 8.1.1 Molecular Mechanisms of Vesicle Budding and Fusion
- 8.2.1 Early Stages of the Secretory Pathway
- 8.3.1 Later Stages of the Secretory Pathway
- 8.4.1 Receptor-Mediated Endocytosis
- 8.5.1 Directing Membrane Proteins to the Lysosome
- 8.6.1 The structure and functions of Lysosomes
- 9.1.1 Brief Introduction of Cell Communication
- 9.2.1 Signaling Molecules and Receptors
- 9.3.1 G Proteins and G Protein-coupled Receptors (GPCR)
- 9.4.1 GPCR Mediated Signaling pathways
- 10.1.1 Outlines of Signaling pathways that control gene activity
- 10.2.1 Phosphoinositide Signaling Pathways
- 10.3.1 TGF-b Receptors and TGFb Signaling Pathways
- 10.4.1 Signaling Pathways controlled by Ubiquitination
- 10.5.1 The RasMAPK and JAKSTAT signaling pathway
- 11.1.1 Actin Filaments
- 11.1.2 Actin Filaments
- 11.2.1 Dynamic Assembly of Actin Filaments
- 11.3.1 Actin-Based Cellular Structures
- 11.4.1 Myosins and Myosin-Powered Movements
- 12.1.1 Microtubule Structure and Organization
- 12.2.1 Microtubule Dynamics
- 12.3.1 Regulation of Microtubule Structure and Dynamics
- 12.4.1 Kinesins & Dyneins Microtubule-Based Motor Proteins
- 12.5.1 Cilia & Flagella Microtubule-Based Surface Structures
- 12.6.1 Mitosis & Mitotic Spindle
- 12.7.1 Intermediate Filaments
- 12.8.1 Coordination & Cooperation Between Cytoskeletal Elements
- 13.1.1 Nuclear Envelope Nuclear Pore Complex & Nuclear Transport
- 13.2.1 Structural Organization of Eukaryotic Chromosomes
- 13.3.1 Morphology and Functional Elements of Eukaryotic Chromosomes
- 13.4.1 Nucleolus
- 14.1.1 Unit 1 Overview of the cell cycle and its control
- 14.2.1 Unit 2 Model Organisms and Methods to Study the Cell Cycle
- 14.3.1 Unit 3 CDK and Its Discovery
- 14.4.1 Unit 4 Regulation of CDK Activity
- 14.5.1 Unit 5 Commitment to the Cell Cycle and DNA Replication
- 14.6.1 Unit 6 Entry into Mitosis and Completion of Mitosis
- 14.7.1 Unit 7 Surveillance Mechanisms in Cell Cycle Regulation
- 14.8.1 Unit 8 Meiosis A Special Type of Cell Division
- 15.1.1 An Overview on Cell-Cell and Cell-Matrix Adhesion
- 15.2.1 Type of Cell Junctions and Anchoring Junctions
- 15.3.1 Tight junctions and Gap junctions
- 15.4.1 The Extracellular Matrix I_the Basal Lamina
- 15.5.1 ECMs of Connective tissue
- 16.1.1 Early metazoan developmnt and embryonic stem cells
- 16.2.1 Stem Cells and Niches in Multicellular Organisms
- 16.3.1 Mechanisms of cell polarity & asymmetric cell division
- 16.4.1 Cell death and its regulation
细胞生物学是主要基础学科,是从细胞的显微、亚显微和分子三个水平对细胞的各种生命活动开展研究的学科。细胞生物学将细胞看作是生命活动的基本单位,在三个不同水平上把组成、结构和功能结合起来,以动态的观点来探索细胞的各种生命活动的具体反应过程。结合分子生物学、生物化学和医学遗传学等学科,通过对细胞生物学的深入研究,可以更加精确地了解生物体的生长、发育、分化、繁殖、运动、遗传、变异、衰老和死亡等基本生命现象。 细胞生物学是研究和揭示细胞结构、功能和生命活动规律的学科。课程内容主要涵盖:生物膜系统,包括细胞质膜与物质跨膜转运、能量转换细胞器与能量合成机制、内膜系统细胞器与蛋白质合成及分选;细胞骨架系统,包括微丝、微管、中间丝及其结合蛋白的结构与功能;遗传信息荷载系统,包括细胞核、染色体及核糖体的结构与功能;细胞重大生命活动及其调控机制,包括细胞增殖与癌细胞、细胞分化与干细胞、细胞衰老与死亡;细胞社会性的结构及生命活动的信号调控网络等。
课程大纲
01
Introduction of Molecular cell biology
课时
1.1.The objectives and development of cell biology
1.2.Microscope and Cell theory
1.3.Major events in cell biology
1.4.Cell theory-test
02
Culturing and visualizing cells
课时
2.1.Growing Cells in Culture
2.2 Monoclonal antibodies
2.3.Fluorescence Microscopy
2.4.Flow cytometry
2.5.Spinning Disk Confocal Microscopy
2.6.FRAP and FRET
2.7.RNA interference
2.8.Culturing and visualizing cells-test
03
Biomembrane Structure
课时
3.1.The structrures of the plasmic membrane
3.2.The lipid bilayer: Composition and structural organization
3.3.Forms of lipid molecules exist in water
3.4.Lipids Movement in biomembrane
3.5.Structure of Membrane Proteins
3.6.Synthesis of Phospholipids and Intracellular Movement
3.7.Biomembrane Structure-test
04
Transmembrane transport of Ions and small molecules
课时
4.1.Overview of transmembrane transport
4.2.Facilitated Transport of Glucose and Water
4.3.ATP –powered pumps
4.4.Na+/k+ pump
4.5.Ca2+ pump
4.6.K+ channel and the Resting membrane potencial
4.7.Cotransport by Symporters and Antiporters
4.8.Transcellular transport
4.9.Transmembrane transport of Ions and small molecules
05
Cellular Energetics
课时
5.1.Overview of aerobic oxidation
5.2.Structure of Mitochondria
5.3.Mitochondrial Oxidation of Fatty Acids Generates ATP
5.4.The Electron Carriers and Electron Transport Chain
5.5.The Electron Transport and Generation of the Proton-Motive Force
5.6.Chemiosmotic theory and Endosymbiont hypothesis
5.7.Harnessing the Proton-Motive Force to synthesize ATP
5.8.Electron Transport and Reactive Oxygen Species (ROS)